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Journal: Annals of Intensive Care
Article Title: Platelet activation in critically ill COVID-19 patients
doi: 10.1186/s13613-021-00899-1
Figure Lengend Snippet: Unbiased transcriptomic analysis reveals primary hemostasis-related genes as upregulated in critically ill COVID-19 patients. RNA from patients of Cohort 2 was extracted from whole blood and RNA counts of 594 genes were determined by direct probe hybridization using the Nanostring nCounter Human Immunology v2 kit. a Gene-set enrichment analysis was performed after ranking genes according to their differential expression in severe vs critical patients. Shown are pathways significantly enriched (false discovery rate < 0.2). b Heatmap representation of genes of the primary hemostasis pathway that are upregulated in a severity grade-dependent manner (determined by hierarchical clustering). Up-regulated genes are shown in red and down-regulated genes in blue. Groups: healthy controls ( n = 13), mild-to-moderate ( n = 11), severe ( n = 10) and critical ( n = 11)
Article Snippet: For gene set enrichment analysis (GSEA), genes were ordered by t -statistic from unpaired t test comparing normalized RNA levels of severe vs critical patients, and then fed to the
Techniques: Hybridization, Quantitative Proteomics